The Human miFinder 384HC miScript miRNA PCR Array profiles the expression of the 372 most abundantly expressed and best characterized miRNAs in miRBase (www.miRBase.org). Each of these miRNAs can regulate one or more messenger RNA transcripts, and conversely a given mRNA can be regulated by one or more miRNAs. Therefore, although they are well-characterized, the complex role played by each miRNA is yet to be completely defined. This array maximizes the likelihood of discovering miRNAs whose expression patterns correlate with the biological phenotypes under study. A set of controls present on this array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green-based real-time PCR, the expression of 372 highly researched miRNAs can be easily and reliably analyzed with this miScript miRNA PCR Array.