TNF Ligand Family: CD40LG (TNFSF5), CD70 (TNFSF7), FASLG (TNFSF6), LTA (TNFB), TNF, TNFSF10 (TRAIL), TNFSF8.
|Apoptosis ChIP PCR Array|
|The Human Apoptosis EpiTect ChIP qPCR Array profiles the histone modification status or “histone code” of 84 key genes involved in programmed cell death. Histone modifications regulate chromatin structure and correlate with the transcriptional activity of associated genes. Apoptosis plays a critical role in normal biological processes requiring cell removal including differentiation, development, and homeostasis. Stress responses (such as heat shock, ischemia, unfolded proteins, and viral infection) cause badly damaged cells to undergo apoptosis. In cell culture, growth factor withdrawal and many known experimental compounds have a similar effect. An acquired defect in apoptosis activation often leads to uncontrolled cell growth, oncogenesis, and cancer. Ligand-bound tumor necrosis factor (TNF) receptors initiate apoptosis by recruiting FADD and other death domain adaptor proteins that then recruit and activate caspases. Environmental stresses trigger BCL2 protein oligomerization and insertion into the mitochondrial membrane, releasing APAF1 and other CARD family members that also oligomerize to recruit and activate caspases. Caspases promote a proteolysis cascade that degrades cellular protein targets, while the IAP protein family directly inhibits caspases. This array includes TNF ligands and their receptors, members of the BCL-2, caspase, IAP, TRAF, CARD, death domain, death effector domain, and CIDE families, as well as genes involved in the p53 and DNA damage pathways. Monitoring the histone modifications of these genes can help determine the epigenetic mechanisms controlling programmed cell death and the propensity of a cell type to undergo apoptosis normally. Using chromatin immunoprecipitation and EpiTect ChIP qPCR Arrays, research studies can easily and reliably analyze the histone modification patterns associated with a focused gene panel related to apoptosis.|
The EpiTect ChIP qPCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease
TNF Receptor Family: CD27 (TNFRSF7), CD40 (TNFRSF5), FAS (TNFRSF6), LTBR, TNFRSF10A, TNFRSF10B (DR5), TNFRSF11B, TNFRSF1A, TNFRSF21, TNFRSF25 (DR3), TNFRSF9.
BCL-2 Family: BAD, BAG1, BAG3, BAG4, BAK1, BAX, BCL2, BCL2A1 (Bfl-1/A1), BCL2L1 (BCL-X), BCL2L10, BCL2L11, BCL2L2, BCLAF1, BID, BIK, BNIP1, BNIP2, BNIP3, BNIP3L, HRK, MCL1.
Caspase Family: CASP1 (ICE), CASP10 (MCH4), CASP14, CASP2, CASP3, CASP4, CASP5, CASP6, CASP7, CASP8 (FLICE), CASP9.
IAP Family: BIRC2 (c-IAP2), BIRC3 (c-IAP1), BIRC6, BIRC8, XIAP.
TRAF Family: TRAF2, TRAF3, TRAF4.
CARD Family: APAF1, BCL10, BIRC2, BIRC3, NOD1 (CARD4), CARD6, CARD8, CASP1 (ICE), CASP2, CASP4, CASP5, CASP9, CRADD, NOL3, PYCARD (TMS1/ASC), RIPK2.
Death Domain Family: CRADD, DAPK1, FADD, FAS (TNFRSF6), TNFRSF10A, TNFRSF10B (DR5), TNFRSF11B, TNFRSF1A, TNFRSF21, TNFRSF25 (DR3), TRADD.
Death Effector Domain Family: CASP8 (FLICE), CASP10 (MCH4), CFLAR (CASPER), FADD.
CIDE Domain Family: CIDEA, CIDEB, DFFA.
p53 and DNA Damage Response: ABL1, AKT1, APAF1, BAD, BAX, BCL2, BCL2L1 (BCL-X), BID, CASP3, CASP6, CASP7, CASP9, GADD45A, TP53, TP53BP2, TP73.
Anti-Apoptosis: AKT1, BAG1, BAG3, BCL2, BCL2A1 (Bfl-1/A1), BCL2L1 (BCL-X), BCL2L10, BCL2L2, BFAR, BIRC2 (c-IAP2), BIRC3 (c-IAP1), BIRC6, BIRC8, BNIP1, BNIP2, BNIP3, BRAF, CASP2, CD27 (TNFRSF7), CFLAR (CASPER), FAS (TNFRSF6), IGF1R, MCL1, NFKB1, TNF, XIA