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Cell Death PathwayFinder PCR Array
The Human Cell Death PathwayFinder RT² Profiler PCR Array profiles the expression of 84 key genes important for the central mechanisms of cellular death: apoptosis, autophagy, and necrosis. Apoptosis, or programmed cell death, results in controlled cell shrinkage and fragmentation via the action of caspases, as well as an anti-inflammatory cytokine release. In contrast, necrosis signals via RIPK1 (RIP1), leading to cell swelling, lysis, and a pro-inflammatory cytokine release. Autophagy destroys the cell's damaged proteins and organelles via an intracellular catabolic process in the lysosome. Multiple cellular processes require the removal of specific cells by a controlled cell-death program. For example, tissue remodeling activates apoptosis, whereas energy metabolism and growth regulation responses rely on autophagy. Developmental processes often activate apoptosis, while bodily injuries or infection more commonly induce necrosis. The molecular mechanisms behind these cell death pathways overlap and more than one form of cell death occur simultaneously during some cellular functions. Apoptosis and necrosis both signal through the death domain receptors FAS, TNFRSF1A (TNFR1), and TNFRSF10A (TRAIL-R), while autophagy and apoptosis share BCL2 family members as key players. The results of this array can yield insights into which central cell death mechanism(s) drive normal biological or pathophysiological processes. Using real-time PCR, research studies can easily and reliably analyze the expression of a focused panel of genes involved in cellular death pathways with this array.
The RT² Profiler PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
96-well Plate, 384-well (4 × 96) Plate, and 100-well Disc formats are available.
澳门威斯尼人手机游戏死亡通路发现者PCR芯片可用于研究参与 澳门威斯尼人手机游戏死亡的中心机制：凋亡、自噬、坏死的84个关键基因的表达。 澳门威斯尼人手机游戏凋亡或程序性 澳门威斯尼人手机游戏死亡有两种情况：半胱氨酸酶和抗炎 澳门威斯尼人手机游戏因子释放，进而导致 澳门威斯尼人手机游戏收缩和破碎；坏死信号通过RIPK1（RIP1）的释放，则导致 澳门威斯尼人手机游戏膨胀和溶解。自噬可以通过 澳门威斯尼人手机游戏内的溶酶体清除 澳门威斯尼人手机游戏中受损的蛋白和 澳门威斯尼人手机游戏器。 澳门威斯尼人手机游戏程序性死亡在生物学过程中时常发生，例如组织重塑会激活 澳门威斯尼人手机游戏凋亡，能量代谢和生长调节也离不开自噬；发育过程会激活 澳门威斯尼人手机游戏凋亡，身体受伤或感染则伴随坏死。凋亡和坏死与FAS、TNFRSF1A（TNFR1）、TNFRSF10A有关；自噬和凋亡则与BCL2家庭有关。使用实时定量PCR，研究者可以很简单和可靠地分析参与 澳门威斯尼人手机游戏死亡通路的基因表达。通过实时定量PCR的方法，研究者即能够利用该芯片简单可靠地同时检测 澳门威斯尼人手机游戏死亡通路相关基因的表达，研究与之相关的正常生理或病理过程的驱动机制
Pro-Apoptotic: ABL1, APAF1, ATP6V1G2, BAX, BCL2L11, BIRC2 (c-IAP2), CASP1 (ICE), CASP3, CASP6, CASP7, CASP9, CD40 (TNFRSF5), CD40LG (TNFSF5), CFLAR (CASPER), CYLD, DFFA, FAS (TNFRSF6), FASLG (TNFSF6), GADD45A, NOL3, SPATA2, SYCP2, TNF, TNFRSF1A, TNFRSF10A (TRAIL-R), TP53.
Anti-Apoptotic: AKT1, BCL2, BCL2A1 (Bfl-1/A1), BCL2L1 (BCL-X), BIRC3 (c-IAP1), CASP2, IGF1R, MCL1, TNFRSF11B, TRAF2, XIAP.
Autophagy: AKT1, APP, ATG12, ATG16L1, ATG3, ATG5, ATG7, BAX, BCL2, BCL2L1 (BCL-X), BECN1, CASP3, CTSB, CTSS, ESR1 (ERa), FAS (TNFRSF6), GAA, HTT, IFNG, IGF1, INS, IRGM, MAP1LC3A, MAPK8 (JNK1), NFKB1, PIK3C3 (VPS34), RPS6KB1, SNCA, SQSTM1, TNF, TP53, ULK1.
Necrosis: ATP6V1G2, BMF, C1orf159, CCDC103, COMMD4, CYLD, DEFB1, DENND4A, DPYSL4, EIF5B, FOXI1, GALNT5, GRB2, HSPBAP1, JPH3, KCNIP1, MAG, OR10J3, PARP1 (ADPRT1), PARP2, PVR, RAB25, S100A7A, SPATA2, SYCP2, TMEM57, TNFRSF1A, TXNL4B.
How it Works
The PCR array is a set of optimized real-time PCR primer assays on 96-well or 384-well plates for pathway or disease focused genes as well as appropriate RNA quality controls. The PCR array performs gene expression analysis with real-time PCR sensitivity and the multi-gene profiling capability of a microarray. Simply mix your cDNA template with the appropriate ready-to-use PCR master mix, aliquot equal volumes to each well of the same plate, and then run the real-time PCR cycling program. (Download user manual)
What it offers?
Layout and Controls: The PCR Arrays are available in both 96- and 384-well plates and are used to monitor the expression of 84 genes related to a disease state or pathway plus five housekeeping genes. Controls are also included on each array for genomic DNA contamination, RNA quality, and general PCR performance
You can easily perform a PCR Array experiment in your own laboratory, or send your samples to us and take advantage of our PCR Array Services.
*: when using complete PCR array system.
Performance Data Sensitivity:
Application DataCancer Research:
To ascertain the oncogenic route that two different human breast tumors have taken, the relative expression level of cancer- and adhesion-related genes in normal and two different cancerous tissues were compared.
Template cDNAs prepared from total RNA of normal human breast and two human breast tumors (BioChain Institute, Inc., 5.0 µg) were characterized in technical triplicates using the Human Cancer PathwayFinder™ PCR Array and the Human Extracellular Matrix & Adhesion Molecule PCR Array with the RT² SYBR Green / Fluorescein PCR master mix on the Bio-Rad iCycler®.
Hepatocellular carcinoma HepG2 cells were treated at 80% cell confluence with these three drugs (100 µM, Cayman Chemical) or a DMSO vehicle control for 24 h. RNA isolated using the ArrayGrade™ Total RNA Isolation Kit was used to characterize gene expression with the Human Drug Metabolism and Stress & Toxicity PathwayFinder™ RT² Profiler™ PCR Arrays and RT² SYBR Green / Fluorescein PCR master mix on the Bio-Rad iCycler®.