科技服务 > PCR芯片实验服务 > Hippo信号通路PCR芯片 Hippo Signaling PCR Array

Hippo信号通路PCR芯片 Hippo Signaling PCR Array

Hippo信号通路PCR芯片 Hippo Signaling PCR Array
运费 ¥0.00
(库存 9999 件)
地区:上海
简介:Hippo Signaling PCR Array
提供商:SAB
服务名称:Hippo Signaling PCR Array
Human Hippo Signaling PCR Array

The Human Hippo Signaling Pathway RT² Profiler PCR Array profiles the expression of 84 genes involved in the Hippo signal transduction cascade. The evolutionarily conserved Hippo pathway was discovered by analyzing Drosophila mutations with overgrown tissues, and mammalian homologues of its components have since been identified and characterized at a molecular level. The Hippo cascade regulates organ size and stem cell properties by governing cell proliferation, apoptosis, and contact inhibition. Alterations of this pathway are frequently found associated with cell overgrowth and tumorigenesis. The Hippo pathway can be divided into upstream regulatory components, the Hippo core kinase components, and the downstream transcriptional machinery for modulating target gene expression. Multiple proteins, some of which mediate cell-cell or cell-ECM adhesion, feed into the core of the Hippo pathway. Upstream regulators such as WWC1, SCRIB, Crumbs complex, cadherins, FAT, and Dachsous modulate the Hippo pathway by activating the core kinase cassette. There, autophosphorylated MST1/2 catalyzes the phosphorylation and activation of LATS1/2 that in turn catalyzes the phosphorylation of the transcriptional co-activators YAP1 and TAZ. In their unphosphorylated states, YAP1 and TAZ freely enter the nucleus and function as transcriptional co-activators. However upon phosphorylation, YAP1 and TAZ instead bind the 14-3-3 family of scaffolding proteins, sequestering them in the cytosol. Upstream regulators can also circumvent the core kinase cassette by sequestering the co-activators at cell-cell junctions instead. This array profiles genes involved in upstream regulation, the core kinase complex, and downstream mediation of the Hippo pathway. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification and assessment of reverse transcription performance, genomic DNA contamination, and PCR performance. Using real-time PCR, research studies can easily and reliably analyze components of the Hippo signaling pathway with this array.
The RT² Profiler PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
96-well Plate, 384-well (4 × 96) Plate, and 100-well Disc formats are available.
 
ProductSpeciesTechnologyCat. No.
Hippo Signaling Pathway PCR ArrayHumanGene ExpressionPAHS-172Z
Hippo Signaling Pathway PCR ArrayMouseGene ExpressionPAMM-172Z
Hippo Signaling Pathway PCR ArrayRatGene ExpressionPARN-172Z




Upstream Regulators: ACTG1, AMOT, AMOTL1, AMOTL2, CASP3, CRB1, CRB2, CRB3, CSNK1D, CSNK1E, DCHS1, DCHS2, DIAPH2, DLG1, DVL2, FAT1, FAT2, FAT3, FAT4, FJX1, AJUBA, LIMD1, LIX1L, LLGL1, LLGL2, LPP, NF2, RASSF2, RASSF4, SCRIB, WTIP, WWC1, ZDHHC18.
Hippo Core Kinase Complex: LATS2, MOB1B, MOB1A, SAV1, STK3, STK4, TAZ, YAP1, MST1, WWTR1.
Downstream Mediators: HIPK2, MAPK10, MEIS1, PRKCI, PRKCZ, SMAD1, TEAD1, TEAD2, TEAD3, TEAD4, TP63, TSHZ1, TSHZ2, TSHZ3, WNT1.
Target Genes: CASP3, CCNE1, CCNE2, MYC, PPP2CB, PPP2R1A, PPP2R2D, PTPN14, RERE.
Contact Inhibition: NF2, TAZ, YAP1.
Cell Polarity: CRB1, CRB2, CRB3, DCHS1, DCHS2, FAT1, FAT2, FAT3, FAT4, LATS1, LATS2, MST1, SCRIB, STK3, STK4, GPC5, HMCN1, POTEF, TAOK1, TAOK2, TAOK3, TJP1, TJP2, MPDZ, MPP5, NPHP4, PARD3, PARD6G.


姓名
电话号码
电子邮箱
公司名称
地址
留言*
 
验证码
提交
我是混淆代码

如果您的浏览器未跳转,请点击此处进行游戏并领取优惠

技术支持 AI智能站群 luis888.vip@gmail.com