WNT Signaling Pathways:
|Human WNT Signaling Pathway DNA Methylation PCR Array, Signature Panel: |
|The Human WNT Signaling Pathway EpiTect Methyl II Signature PCR Array profiles the promoter methylation status of a panel of 22 promoters of genes involved in WNT signaling during carcinogenesis and cellular differentiation. Hypermethylation of WNT pathway inhibitory proteins (NKD1 and WIF1) causes activation of WNT signaling as well as uncontrolled cell proliferation, a hallmark of cancer. Hypermethylation of WNT ligand genes (such as WNT10B) down-regulates expression during differentiation (e.g., adipogenesis). Profiling cellular or fresh tissue genomic DNA samples with these arrays may help correlate CpG island methylation status with biological phenotypes. The results may also help provide further insights into how WNT signaling contributes to carcinogenesis and cellular differentiation. With a simple restriction enzyme digestion and real-time PCR, research studies can analyze the promoter methylation status of 22 different WNT signaling pathway members with this DNA methylation PCR array.|
Both 96-well and 384-well ( 4 X 96 ) formats are available
Canonical: CTBP1, CXXC4, FZD1, FZD2, FZD4, FZD5, FZD8, FZD9, GSK3A, GSK3B, LEF1, NKD1, SENP2, SFRP4, SOX17, WIF1, WNT1, WNT10A, WNT10B, WNT6.
Planar Cell Polarity (PCP): NKD1, WNT9A.
WNT/Ca+2: FZD2, WNT1, WNT10A, WNT10B, WNT5A, WNT6, WNT9A.
WNT Signaling Negative Regulation: CTBP1, CXXC4, NKD1, SENP2, SFRP4, SOX17, WIF1.
WNT Signaling Components:
Ligands & Cofactors: SFRP4, WIF1, WNT1, WNT10A, WNT10B, WNT5A, WNT6, WNT9A.
Receptors: FZD1, FZD2, FZD4, FZD5, FZD8, FZD9.
Downstream Signaling: CXXC4, GSK3A, GSK3B, NKD1, SENP2, SOX17.
Transcription Factors & Cofactors: CTBP1, LEF1.